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. 2013 May 1;12:40. doi: 10.1186/1475-2859-12-40

Figure 1.

Figure 1

Metabolic pathways for the whole cell biosynthesis of 2′-fucosyllactose (2′-FL) in Escherichia coli. Salvage GDP-L-fucose pathway enzyme is depicted in red, de novo GDP-L-fucose synthesis pathway enzymes in blue and α1,2-fucosyltransferase enzyme in green. Enzymes and molecules are abbreviated as follows: Gal, D-galactose; GDP-4k-6d-Man, GDP-4-keto-6-deoxymannose; GDP-Man, GDP-α-D-mannose; GDP-L-Fuc, GDP-β-L-fucose; Glc, D-glucose; FucP, fucose permease; GlpF, glycerol MIP channel; LacY, lactose permease; Man-1P, α-D-mannose-1-phosphate; Man-6P, α-D-mannose-6-phosphate; ManB, phosphomannomutase; ManC, mannose-1-phosphate guanylyltransferase; Gmd, GDP-mannose 4,6-dehydratase; WcaG, GDP-fucose synthase; FutC, α1,2-fucosyltransferase; LacZ, β-galactosidase.