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HPLC analysis of intracellularly accumulated GDP-L-fucose. A) Cell extract of E. coli JM109 gwBC-F1-fkp 12h after induction. B) Cell extract of E. coli JM109 gwBC-F1 12h after induction. C) Cell extract of E. coli JM109 12h after induction. Arrows indicate retention time of GDP-L-fucose and GDP, respectively.
