Table 1.
Bacterial strains and plasmids used in this study
| Strains | Relevant genotype or sequences | Source or reference |
|---|---|---|
|
E. coli DH5α |
F-, ϕ80d, lacZΔM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, Δ(lacZYA-argF)U169 |
laboratory strain |
|
E. coli JM109 |
F′ traD36 proA+B+lacIqΔ(lacZ)M15/Δ(lac-proAB) glnV44 e14-gyrA96 recA1 relA1 endA1 thi hsdR17 |
laboratory strain |
|
E. coli JM109 gwBC |
E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG |
this study |
|
E. coli JM109 gwBC-F1 |
E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC |
this study |
|
E. coli JM109 gwBC-F1-fkp |
E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK ::fkp |
this study |
|
E. coli JM109 gwBC-F2 |
E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK ::futC |
this study |
|
E. coli JM109 gwBC-F2-fkp |
E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK::futC, araBAD::fkp |
this study |
|
Plasmids |
|
|
| pKD46 |
ParaB γ β exo (red recombinase), AmpR |
[35] |
| pCP20 |
FLP+, λ cI857+, λ pR Repts, AmpR, CmR |
[34] |
| pMJC54 |
pET16b, B. fragilis fkp gene, AmpR |
[17] |
| pCAW48 |
pET11a, S. enterica manB gene, AmpR |
Albermann & Piepersberg (unpublished) |
| pCAW49 |
pET11a, S. enterica manC gene, AmpR |
Albermann & Piepersberg (unpublished) |
| pCAW55 |
pJOE2775, H. pylori futC gene, AmpR |
[14] |
| pCAS30-FRT-cat-FRT |
pJF119ΔN, P. ananatis crtE gene, FRT-sites, AmpR, CmR |
[36] |
| pJF-crtY-FRT-kan-FRT |
pJF119ΔN, P. ananatis crtY gene, FRT-sites, AmpR, KanR |
[32] |
| pQE31-FRT-cat-FRT |
pQE31, FRT-sites, AmpR, CmR |
[37] |
| pJF119ΔN |
cloning vector, RBS, Ptac, AmpR |
[38] |
| pJF-manB |
pJF119ΔN, S. enterica manB gene, AmpR |
this study |
| pJF-manC |
pJF119ΔN, S. enterica manC gene, AmpR |
this study |
| pJF-gmdwcaG |
pJF119ΔN, E. coli gmd, wcaG gene, AmpR |
this study |
| pJF-futC |
pJF119ΔN, H. pylori futC gene, AmpR |
this study |
| pJF-fkp |
pJF119ΔN, B. fragilis fkp gene, AmpR |
this study |
| pJF-manB-FRT-cat-FRT |
pJF119ΔN, S. enterica manB gene, FRT-sites, AmpR, CmR |
this study |
| pJF-manC-FRT-kan-FRT |
pJF119ΔN, S. enterica manC gene, FRT-sites, AmpR, KanR |
this study |
| pJF-gmdwcaG-FRT-cat-FRT |
pJF119ΔN, E. coli gmd, wcaG gene, FRT-sites, AmpR, CmR |
this study |
| pJF-futC-FRT-cat-FRT |
pJF119ΔN, H. pylori futC gene, FRT-sites, AmpR, CmR |
this study |
| pJF-fkp-FRT-cat-FRT | pJF119ΔN, B. fragilis fkp gene, FRT-sites, AmpR, CmR | this study |