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Protein production after a freezing step of the mycelium. Amylase (A) and Xylanase (C) production by S. lividans ΔTA-pKC796-Tox transformed with pNAnti-Amy (A) and pNAnti-Xyl (C) before and after freezing the mycelium. 10 μL of 4-day supernatants was loaded in each track. B and D show the percentages of amylase (B) and xylanase (D) activity of the supernatants. The histogram bars are the mean of four experiments.
