Table 2.

Plasmids used

Plasmid	Characteristics	Reference
pGM160	E.coli/Streptomyces shuttle vector. Thiostrepton and gentamicin resistance.	[24]
pGM160-YefMsl	pGM160 derivative. The xysA promoter from S. halstedii controls yefMsl expression.	[23]
pKC796	E.coli/Streptomyces shuttle vector. Apramycin resistance. Integrative plasmid.	[30]
pKC796-Tox	pKC796 derivative. The xysA promoter from S. halstedii controls toxin expression.	[23]
pKC796-TA	pKC796 derivative. The xysA promoter from S. halstedii controls TA expression.	[23]
pXHis1	pBluescript SK derivative. Ampicillin resistance. The xysA promoter from S. halstedii controls xys1Δ expression.	[28]
pXHis1-Anti	pXHis1 derivative. The xysA promoter from S. halstedii controls antitoxin expression.	This work
pN702Gem3	E.coli/Streptomyces shuttle vector. Neomycin resistance. High-copy number.	[29]
pN702Gem3-Anti	pN702Gem3 derivative. The xysA promoter from S. halstedii controls antitoxin expression.	This work
pNUF5	pN702Gem3 derivative. The pstS promoter from S. lividans controls xylanase expression.	[31]
pNUF-Amy	pNUF5 derivative. The pstS promoter from S. lividans controls amylase expression.	This work
pNAnti-Xyl	pN702Gem3-Anti derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls xylanase expression.	This work
pNAnti-Amy	pN702Gem3-Anti derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls amylase expression.	This work