Table 4.
Sequence details of all primer-probe combinations
|
Virus type |
Target |
Amplicon |
Oligonuleotide sequence(5′-3′) |
Nucleotide positions |
GenBank accession no. |
|---|---|---|---|---|---|
| |
|
length(bp) |
Forward |
|
|
| |
|
|
Reverse |
|
|
| Probe | |||||
| Parainfluenza 1 |
HN gene |
109 |
GTTGTCAATGTCTTAATTCGTATCAATAATT |
1191–1220 |
U70948 |
| |
|
|
GTAGCCTACCTTCGGCACCTAA |
1278–1299 |
|
| |
|
|
TTGGAATAGTCTCGACAACAATCTTTGGCCTA |
1232–1263 |
|
| Parainfluenza 2 |
HN gene |
90 |
GCATTTCCAATCTTCAGGACTATGA |
767–791 |
D00865 |
| |
|
|
ACCTCCTGGTATAGCAGTGACTGAAC |
831–856 |
|
| |
|
|
CCATTTACCTAAGTGATGGAATCAATCGCAAA |
795–826 |
|
| Parainfluenza 3 |
HN gene |
136 |
AGTCATGTTCTCTAGCACTCCTAAATACA |
779–807 |
L25350 |
| |
|
|
ATTGAGCCATCATAATTGACAATATCAA |
887–914 |
|
| |
|
|
AACTCCCAAAGTTGATGAAAGATCAGATTATGCA |
828–861 |
|
| Respiratory syncytial virus |
F gene |
90 |
AACAGATGTAAGCAGCTCCGTTATC |
789-813 |
AF067125 |
| |
|
|
CGATTTTTATTGGATGCTGTACATTT |
853–878 |
|
| |
|
|
TGCCATAGCATGACACAATGGCTCCT |
822-847 |
|
| Human metapneumovirus |
N gene |
163 |
CATATAAGCATGCTATATTAAAAGAGTCTC |
89-116 |
AB503857 |
| |
|
|
CCTATTTCTGCAGCATATTTGTAATCAG |
224–251 |
|
| |
|
|
TGCAATGATGAGGGTGTCACTGCGGTTG |
149-176 |
|
| |
|
|
TGTAATGATGAGGGTGTCACTGCGGTTG |
|
|
| Adenoviru A |
Hexon gene |
135 |
GGTCTGGTGCAATTCGCC |
17818–17835 |
X73487 |
| |
|
|
CACGGGCACAAAACGCA |
17936–17952 |
|
| |
|
|
CCACGGACACCTACTTCACCCTGGG |
17840–17864 |
|
| Adenoviru B |
Hexon gene |
138 |
CGCCGGACAGGATGCTT |
45–61 |
X76549 |
| |
|
|
CTACGGTCGGTGGTCAC |
166–182 |
|
| |
|
|
AGTCCGGGTCTGGTGCAGTTCGCC |
73–96 |
|
| Adenoviru C |
Hexon gene |
143 |
ACCTGGGCCAAAACCTTCTC |
2884–2903 |
J01966 |
| |
|
|
CGTCCATGGGATCCACCTC |
2940–2958 |
|
| |
|
|
AACTCCGCCCACGCGCTAGA |
2910–2929 |
|
| Adenoviru D |
VA gene |
75 |
AAAAACGAAAGCGGTTGAGC |
2–21 |
U10675 |
| |
|
|
CGGGTCGAGACGGGAGT |
128–144 |
|
| |
|
|
CCAATACCACGTTAGTCGCGGCT |
104–126 |
|
| Adenoviru E |
Hexon gene |
113 |
CAACACCTACTCGTACAAAGTGCG |
225–248 |
X84646 |
| |
|
|
TAGGTGCTGGCCATGTCCA |
281–299 |
|
| |
|
|
CGCCCACGGCCAGCGTGT |
251–268 |
|
| Adenoviru F |
1.5 χ-2 gene |
|
CCCGTGTTTGACAACGAAGG |
31277–31296 |
L19443 |
| |
|
|
TTAGAGCTAGGCATAAATTCTACAGCA |
31363–31389 |
|
| ATCGACAAGGACAGTCTGCCAACACTAACG | 31326–31355 |