Figure 2.

Effects of the growth temperature on HSA secretion. Culture samples, grown at 5 - 40°C, have been analyzed both at the growth and stationary phases. A) Western blot analysis of secreted rHSA; B) Western blot analysis of intracellular rHSA; C) Q-PCR analysis of rHSA mRNA. Data are reported as a function of growth phase and temperature. The values, means of three independent experiments ± standard deviations, have been normalized setting at 1 the highest value. Superscripts indicate statistically similar means (P > 0.05). Symbols are used to compare sample grown at the same temperature, collected at different growth phases (exponential or stationary). Letters are used to compare samples collected at the same growth phase, cultured at diverse temperatures.