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Cloning strategy for the construction of pPICZαChU-B, pGAPZαChU-B, pPICZα*ChU-B and pGAPZα*ChU-B plasmids. The pJRoC30α*ChU-B was used as template to amplify ChU-B with/without the evolved α-factor prepro-leader (α*) from S. cerevisiae. See Methods section for details.
