. 2013 Apr 30;13:38. doi: 10.1186/1472-6750-13-38

Table 4.

Steady-state kinetic parameters of ChU-B mutant expressed in P. pastoris and S. cerevisiae

Substrate	pH	*ChU-B fromP. pastoris***			*ChU-B fromS. cerevisiae***			(Parental type, OB-1mutant^a)
Substrate	pH	K_m(mM)	k_cat(s^-1)	k_cat/K_m(mM^-1s^-1)	K_m(mM)	k_cat(s^-1)	k_cat/K_m(mM^-1s^-1)	K_m(mM)	k_cat(s^-1)	k_cat/K_m(mM^-1s^-1)
ABTS	3.0	0.024 ± 0.002	22.3 ± 0.5	929	0.023 ± 0.001	57.0 ± 0.9	2478	0.0063 ± 0.0009	200 ± 7	31746
ABTS	7.4	0.26 ± 0.01	1.13 ± 0.02	4.35	0.32 ± 0.02	3.09 ± 0.07	9.66	n.d.	n.d.	n.d.
DMP	5.0	1.91 ± 0.05	5.65 ± 0.05	2.96	2.0 ± 0.2	19.5 ± 0.5	9.75	0.14 ± 0.02	134 ± 5	957
DMP	7.4	0.23 ± 0.03	0.41 ± 0.02	1.78	0.22 ± 0.03	1.95 ± 0.05	8.86	n.d.	n.d.	n.d.

^a Data from Maté et al., 2010. n.d. not determined.