Table 2. Plasmids used in this study. All the plasmids carry Ampicillin resistance marker and the cloned gene expresses from a T7 promoter, when an appropriate host is used.

Plasmids	Size (kbp)	Description	Source
pSK01-NCHS	5.8	∼500 bp orf sandwiched between N- & C-ter His6 tag	Kind gift of Soumya Kamilla, IISER Bhopal
pMS-QS-NOHS	5.3	No His6 tag is expressed along with the gene	This study
pMS-QS-CHS	5.3	C-ter His6 tag is expressed along with the gene	This study
pMS-QS-NHS	5.4	N-ter His6 tag is expressed along with the gene	This study
pMS-QS-NOHGFP	6.0	GFP expression without any tag	This study
pMS-QS-CHGFP	6.0	GFP expression with His6 tag at its C-ter	This study
pMS-QS-NHGFP	6.1	GFP expression with His6 tag at its N-ter	This study
pMS-QS-NOHSigB	6.3	H37Rv Sigma factor B expression without any tag	This study
pMS-QS-CHSigB	6.3	H37Rv Sigma factor B expression with His6 tag at its C-ter	This study
pMS-QS-NHSigB	6.4	H37Rv Sigma factor B expression with His6 tag at its N-ter	This study
pMS-QS-NOHSigD	5.9	H37Rv Sigma factor D expression without any tag	This study
pMS-QS-CHSigD	5.9	H37Rv Sigma factor D expression with His6 tag at its C-ter	This study
pMS-QS-NHSigD	6.0	H37Rv Sigma factor D expression with His6 tag at its N-ter	This study
pMS-QS-NOHSigF	6.1	H37Rv Sigma factor F expression without any tag	This study
pMS-QS-CHSigF	6.1	H37Rv Sigma factor F expression with His6 tag at its C-ter	This study
pMS-QS-NHSigF	6.2	H37Rv Sigma factor F expression with His6 tag at its N-ter	This study
pMS-QS-NOHSigG	6.4	H37Rv Sigma factor G expression without any tag	This study
pMS-QS-CHSigG	6.4	H37Rv Sigma factor G expression with His6 tag at its C-ter	This study
pMS-QS-NHSigG	6.5	H37Rv Sigma factor G expression with His6 tag at its N-ter	This study