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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Gene Ther. 2013 Feb 7;20(8):868–873. doi: 10.1038/gt.2013.6

Figure 2. Identification of FV integration sites.

Figure 2

(a) Diagram of an integrated reprogramming vector with locations of the LTR probe shown. A, Avr II sites. (b) Southern blot analysis of Avr II-digested genomic DNAs to determine the number of FV vector integration sites in each iPSC clone. Each integrant produces 2 LTR-hybridizing fragments. (c) Inverse PCR strategy for identifying chromosome-provirus junctions. R, restriction enzymes sites; open arrows, LTR-specific PCR primers; jagged box, LTR remnant; closed arrow, sequencing primers.