Skip to main content
. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Mol Biol. 2013 Mar 13;425(11):2056–2071. doi: 10.1016/j.jmb.2013.03.001

Fig. 4.

Fig. 4

Most constructs react with anti-gB monoclonal antibody DL16, specific for the postfusion form. (A) Mutants designed to stabilize the prefusion conformation. (B) Mutants designed to destabilize the postfusion conformation. Proteins secreted into the supernatant were detected by a Western blot with anti-gB monoclonal antibodies DL16 (top panels) or C226 (bottom panels). Under nonreducing mildly denaturing conditions, gB migrates as a trimer. T – trimer, M – monomer. Positions of molecular weight standards are labeled. In A, lanes are numbered and corresponding constructs are listed underneath the blots. “L6” stands for linker sequence GSGTGS; “L10” stands for linker sequence GGSGGTGGSG. “Cyto” corresponds to the entire gB cytoplasmic domain. In B, lanes are labeled at the top.