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. 2013 May 15;8(5):e63464. doi: 10.1371/journal.pone.0063464

Figure 4. Control of Mycobacterium bovis growth control in bovine macrophages is related to nitric oxide production.

Figure 4

Adherent-macrophages stimulated or not with purified E. coli 026:B6 LPS (100 ng/mL) for 22 h, were infected with M. bovis field strains 9926 or 129QP (MOI of 10) for 4 h, washed and cultured again for 24 h in presence or absence of n G-monomethyl-L-arginine monoacetate (MMLA). Bacterial intracellular growth was calculated by dividing the number of intracellular CFU at 24 h postinfection by the number of intracellular CFU at the start of the assay and expressed as percentage. Values are mean ± standard deviation of triplicates of two independent experiments with macrophages of three susceptible and three resistant cows and challenged with each field strain. Statistical differences (P<0.05) of each bar with its respective control (no treatment) (*) and among phenotypes on each condition (‡) are indicated.