Figure 7. Mycobacterium bovis bovine macrophage apoptosis induction is nitric oxide independent.

Adherent-macrophages stimulated or not with purified E. coli 026:B6 LPS (100 ng/mL) for 22 h, were infected with M. bovis field strain 9926 (MOI of 10) for 4 h, washed and cultured again for 24 h in presence or absence of n ^G-monomethyl-L-arginine monoacetate (MMLA) and stained with TUNEL (BrdUTP-FITC). Values are percentages of true BrdU-FITC TUNEL-positive cells of one experiment, representative of two independent experiments with macrophages of three susceptible and three resistant cows. Kruskal-Wallis test showed significant variation versus uninfected controls (P<0.05) but not between treatments (P = 0.4373) regardless of macrophage phenotype.