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| No. of gel | Cy2 | Cy3 | Cy5 |
| 1 | Internal standard | S phase (1) | E G2/M phase (1) |
| 2 | Internal standard | L G2/M phase (1) | G1 phase (1) |
| 3 | Internal standard | S phase (2) | L G2/M phase (2) |
| 4 | Internal standard | G1 phase (2) | E G2/M phase (2) |
| 5 | Internal standard | G1 phase (3) | S phase (3) |
| 6 | Internal standard | E G2/M phase (3) | L G2/M phase (3) |
