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. 2013 May 16;4:127. doi: 10.3389/fpls.2013.00127

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Copyright © Zhang, Hanisch, Kwaaitaal, Pedersen and Thordal-Christensen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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HvSec61βa-GFP localizes around the Bgh haustorial body. Confocal image of an epidermal cell, transformed with the HvSec61βa-GFP construct, taken 48 h after Bgh inoculation. (A–C) Three different focal planes from an image series of an infected cell with a haustorium. HvSec61βa-GFP localizes to the ER around the nucleus (arrow head, A) and surrounds the haustorium in an ER-like tubular pattern (asterisk, A). (C–E) GFP fluorescence (C), bright field (BF) (D) and merged image (E) show HvSec61βa-GFP localization at the surface of the haustorial body. HvSec61βa-GFP labels the tubular ER network, which is further illustrated in the 3D projection (F) (Image Surfer 1.2). Scale bar, 10 μm.