Figure 7.

In vitro recombination assay using the GFPi-CFP variant. (A) Linear representation of the GFPi-CFP reporter. (B) The recombination of different RSSs pairs was determined upon 293T cells transfection with each GFPi-CFP variants together with the CMV-RAG1/2 expressing plasmids. The 23-RSS Con23 and MI-conPI(19,20), as well as the 12-RSS Con12 and LMO2 were as in Figure 5. Wu-Con12 is a consensus 12-RSS defined by bio-informatics (Ramsden et al., 1994). The 3′Dδ3 is a physiological partner of LMO2 described in Dik et al. (2007). The specific pairs of RSS tested are indicated above the corresponding FACS plots and the sequences listed in Table 2. The recombination efficiency defined as (% GFP⁺ cells in test -% GFP⁺ cells in control) is indicated in the upper part of each plot. The relative recombination coefficient ρ, when con12/con23 is 1, is indicated in the bottom part of each plot, in blue. four replicates of two independent experiments.