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. 2013 May 16;3:1852. doi: 10.1038/srep01852

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(a) Comparison of detection efficiencies between differently labeled detection probes. Alexa Fluor 647, Atto 647N. (b) Mean fluorescence intensity values of 5 kbp DNA fragment using Alexa 647 labeled detection probes. Concentration of target fragments in spiked samples introduced in the assay, final concentration while incubation phase. (c) Visualization of increasing bead brightness with ascending target concentration using the Atto 647N label. Dotted lines display the mean blank sample intensity level; error bars represent the standard error of mean bead intensities (SEM, n = 53–97). Solid lines are guides to the eye. Error bars of (a) indicate the standard error of mean intensity (SEM) between three independent measurements demonstrating the reliability of the parameter.

Inline graphic — (a) Comparison of detection efficiencies between differently labeled detection probes. Alexa Fluor 647, Atto 647N. (b) Mean fluorescence intensity values of 5 kbp DNA fragment using Alexa 647 labeled detection probes. Concentration of target fragments in spiked samples introduced in the assay, final concentration while incubation phase. (c) Visualization of increasing bead brightness with ascending target concentration using the Atto 647N label. Dotted lines display the mean blank sample intensity level; error bars represent the standard error of mean bead intensities (SEM, n = 53–97). Solid lines are guides to the eye. Error bars of (a) indicate the standard error of mean intensity (SEM) between three independent measurements demonstrating the reliability of the parameter.