Table 1. Sequences of the used oligonucleotides. Letters in bold indicate LNA nucleotides.
Description/Name | Sequence from 5′ to 3′ |
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Capture Strand | Amino modifier C6-AAAAAAAAAA- CCGGCGAGGAGTTCGGCCACTACCG |
Detection Strand | Fluorophore-GATGATCACCAAGGACGGCGCCTCC |
Blocking Oligomer 1 | GATCCCCCGCCTGGA |
Blocking Oligomer 2 | TCGACTACACCGCCCCCGACTTCGC |
Blocking Oligomer 3 | CCGCCAGCGGGCCTTCTACGAGGCC |
Blocking Oligomer 4 | CTTTTCGGTTGGACCTTCACCGACT |
Blocking Oligomer 5 | CGCCCCGCCCGCCGCGTGGAC |
150 bp target fragment | GCGCCGCCCACGGAGGCGCCGTCCTTGGTGATCATCCGGTAGTGGCCGAACTCCTCGCCGGAGTCGGTGAAGGTCCAACCGAAAAGGGCCTCGTAGAAGGCCCGCTGGCGGGCGAAGTCGGGGGCGGTGTAGTCGATCCAGGCGGGGGATC |