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. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: Protein Eng Des Sel. 2011 Oct 13;24(12):873–881. doi: 10.1093/protein/gzr049

Fig. 4.

Fig. 4

Chain shuffling of the GFP+ population. The VH and VL genes from the GFP+ population of Fig. 3A were amplified by PCR, then assembled at random to generate a new shuffled library. This “Shuffled library” was analysed by FACS and compared to the untransfected HeLa cells and the GFP+ population.