Fig. 5.

Schematic view of the in vivo folding fate of scFv-GFP fusions. In this scheme, the fusion protein may follow folding (fold), aggregation (agg) and degradation (deg) pathways. The kinetic competition between the pathways are supposed to be affected by the cellular environment, represented here by molecular chaperones (chaperones). Degradation and aggregation are though to proceed from the same or a closely-related misfolded intermediate (misfold). Both native protein and aggregates are fluorescent. The relative magnitudes of the fluxes, estimated from the Western blots in Fig. 1C & 2A, are given at the right of the figure, both for the untagged and the EGFP-tagged aggregation-prone scFv2F12 expressed at high levels using transient transfection.