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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: J Immunotoxicol. 2011 Dec 1;9(2):129–140. doi: 10.3109/1547691X.2011.631953

Figure 4.

Figure 4

Isotypes secreted by CH12.LX cells incubated in media from RAW264.7 macrophages treated with asbestos (35 μg/cm2) or left untreated. Data was normalized to reflect amount of antibody produced by each cell. CH12.LX cells were incubated for 72 h and the concentrations of individual isotypes was then measured using an ELISA. MΦ Media w/o Asbestos connotes antibody production by CH12. LX cells cultured in media from unexposed macrophages; MΦ Media w/Asbestos connotes antibody production by CH12.LX cells cultured in media from macrophages exposed to asbestos. n = 3; error bars = SEM. Treatment with asbestos caused the CH12.LX B-lymphocytes to significantly (*p < 0.05) increase secretion of (a) IgG1, (b) IgG3, and (c) IgA, but not (d) IgM.