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Isotype ELISA data depicting levels of antibody secreted by CH12.LX B-lymphocytes incubated for 48 h with TNFα. Data was normalized to reflect amount of antibody produced by each cell. TNFα was unable to stimulate the CH12.LX cells to increase production of the three isotypes (a) IgM, (b) IgG, or (c) IgA. n = 3; Error Bars = SEM.
