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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Neuropsychopharmacology. 2009 Jun 17;34(10):2344–2357. doi: 10.1038/npp.2009.64

Figure 5. Memantine was neuroprotective against LPS-induced neurotoxcity.

Figure 5

(A) Rat primary neuron-glia cultures were pretreated with memantine (0.1–10 µM) for 30 min before LPS (5 ng/ml) administration. [3H] DA uptake was determined seven days after LPS stimulation. (B) Neuron-enriched cultures were supplemented with 7.5 × 104/well of enriched microglia. Twenty-four hrs later, this neuron-microglia co-cultures were pre-treated with memantine (10 µM) for 30 min before LPS (2.5 ng/ml) administration. [3H] DA uptake was determined seven days after LPS stimulation. Results were expressed as a percentage of the vehicle-treated control cultures and were the mean ± S.E.M. from four independent experiments in triplicate. *, p<0.05, versus control; #, p<0.05 and ##, p<0.01, ###, p<0.001 versus LPS-treated. (C) Examples of control, LPS without or with memantine-treated neurons stained by TH antibody. Upper panel: neuron-glia cultures; lower panel: neuron-microglia co-cultures. (D) Examples of OX42 staining for activated microglia in neuron-glia cultures. (E) Examples of OX42 staining for activated microglia in neuron-microglia co-cultures.