Skip to main content
. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Neuropsychopharmacology. 2009 Jun 17;34(10):2344–2357. doi: 10.1038/npp.2009.64

Figure 6. Memantine inhibited LPS-induced production of reactive oxygen species (ROS), and proinflammatory factors.

Figure 6

(A, B) Microglia-enriched cultures seeded at 5 × 104/well in 96-well plates were pre-treated with memantine (10 µM) for 30 min before LPS (5 ng/ml) administration. (A) Production of extracellular superoxide was measured as SOD-inhibitable reduction of WST-1. Results were normalized to percentage of control level. (B) iROS production was determined by a fluorescence probe DCFH-DA. (C–E) Rat primary midbrain neuron-glia cultures were pre-treated with vehicle and memantine (1–10 µM) for 30 min before the LPS (5 ng/ml) stimulation. Supernatant was collected at 4 h for TNF-α assay (C), at 24 h for nitrite assay (D) and PGE2 assay (E). Results were expressed as mean ± S.E.M. from three 36 independent experiments in triplicate. #, p<0.05 and ##, p<0.01 versus LPS-treated cultures. *, p<0.05, versus control.