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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Cell Rep. 2013 Jan 31;3(2):401–410. doi: 10.1016/j.celrep.2013.01.007

Figure 2. Circulating Nonneuronal Nonautonomous Factors Drive b Cell Replication in LIRKO Mouse.

Figure 2

(A) Schematic of the parabiosis experiment. See also Figures S2, S3, and S4.

(B–E) Single and parabiont models were intraperitoneally injected with BrdU (100 mg/kg body weight) 5 hr before animals were sacrificed, and pancreases were dissected and immunostained for insulin, BrdU, and DAPI.

(F) Quantification of BrdU+ insulin+ cells: three sections separated by 80 µm were analyzed, and between 2,000 and 10,000 cells were counted in each group (n = 5–6 in each parabionts group).

(G) Schematic of the transplantation experiment.

(H) Quantification of BrdU+ insulin+ cells in islet grafts as indicated: three to six islet graft sections were analyzed and counted between 2,000 and 10,000 cells in each group (n = 3–5 in each group).

Data represent mean ± SEM. *p ≤ 0.05.