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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Cell Rep. 2013 Jan 31;3(2):401–410. doi: 10.1016/j.celrep.2013.01.007

Figure 4. LIRKO Serum Increases Mouse and Human Islet β Cell Replication In Vitro.

Figure 4

Five to 6-week-old mouse islets were stimulated with control or LIRKO serum for 48 hr. Islets were embedded in agarose and used for immunostaining studies. Culture media were assayed for glucose and insulin. WT, wild-type.

(A) Schematic of the experimental protocol. See also Figures S5 and S6.

(B) Representative images of mouse islets stimulated with sera derived from 3-month-old (upper panel) and 12-month-old animals (lower panel).

(C) Quantification of Ki67+ insulin+ cells in (B): two sets of three serial sections separated by 80 µm were analyzed. At least 4,000–5,000 cells were counted in each experimental group (n = 5 in each group).

(D) Quantification of TUNEL+ insulin+ cells in (B): at least 3,000–4,000 cells were counted in each group (n = 5 in each group).

(E and F) Representative images of healthy and type 2 diabetic donor islets stimulated with control versus LIRKO serum for 24 hr. See also Table S3.

(G) Quantification of Ki67+ insulin+ cells in (E) and (F): three sets of three serial sections separated by 80 µm were analyzed. At least 3,000–4,000 cells were counted in each group. See also Table S3.

Data represent mean ± SEM. *p ≤ 0.05. (See Serum Stimulation and Human Islet Studies sections in Experimental Procedures.)