Skip to main content
. 2013 Apr 24;2013:326986. doi: 10.1155/2013/326986

Figure 3.

Figure 3

Changes in p-Akt/Akt induced by PAN, PAN + DEX, and LY294002 in podocytes. (a) p-Akt/Akt after 0, 15, 30, 45, 60, and 120 min of PAN (50 μg/mL). (b) Concentration-dependence of PAN-induced changes in p-Akt/Akt as measured at 15 min after treatment with 0, 12.5, 25, 50, 70, or 100 μg/mL PAN. (c) Reversal of the PAN response by DEX co-treatment and effect of LY294002 at 30 min. Lane 1: Control group, Lane 2: DMSO (0.02%), Lane 3: PAN (50 μg/mL), Lanes 4–6: PAN (50 μg/mL) + DEX (0.1, 1, or 10 μmol/L), Lane 7: Pretreatment with LY294002 for 1 hour + PAN (50 μg/mL) + DEX (10 μmol/L) for 30 min. Lysates from podocyte treatment groups were collected for Western blot examination to detect the expression of p-Akt and total Akt. *P < 0.05 and **P < 0.01 compared to the control group; # P < 0.05 compared to PAN-treated group; in comparison with DEX-treated group *, # P < 0.01.