Figure 2.

Loss of STING leads to complete abrogation of CMA-induced cytokine production. (A) Bone marrow-derived wild-type and STING-deficient macrophages were stimulated with LPS and CMA (500 μg/ml). Phospho-IRF3 (P-IRF3) and phospho-NF-κB-p65 (P-NF-κB p65) were assessed at indicated time points (in minutes) after stimulation. (B) Bone marrow-derived wild-type and STING-deficient macrophages were transfected with poly(I:C), pppRNA, ISD and c-diGMP, or stimulated with LPS and CMA (500 or 250 μg/ml) in duplicates. Supernatants were collected after 18 h, and IP-10 levels were measured by enzyme-linked immunosorbent assay. Representative results out of two independent experiments are depicted, whereas data are presented as mean values+s.e.m.

Source data for this figure is available on the online supplementary information page.

Source Data Files for Figure 2 ^{(38MB, zip)}