FIG. 1.

Expression of the transgene in spa/spa mice hetero- and homozygous for the TG 456 insertion. (A) Northern analysis. Total brain RNA was probed with a 264-bp SspI–NcoI fragment encompassing exons 4 and 5 of the GlyR β cDNA. Control hybridizations of the same blot were performed using the GAPDH gene probe. wt, wild-type control mice. (B) Western analysis. Membrane proteins from spinal cord and brain stem were separated on SDS–PAGE gels, transferred to nitrocellulose, and specific bands were detected with the antibodies mAb 4a and anti-SVP against synaptophysin as a control. Immunoglobulins were visualized using horseradish peroxidase-coupled second antibodies and the ECL system (Amersham).