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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Immunol Lett. 2012 Jan 21;143(1):85–91. doi: 10.1016/j.imlet.2012.01.003

Fig. 4. Anti-DNA antibody 8D8 preferentially binds plasmid fragments, but not CG50 of CpG island clone 11 fragments.

Fig. 4

The anti-DNA antibody 8D8 was immobilized on Protein G sepharose and incubated with 32P-labeled DNA fragments resulting from restriction enzyme digestion of LITMUS-CG50 (A), pCpG-mcs-CG50 (B), or pCpG-mcs-clone 11 (C). Sequential salt washes (shown on the x-axis) were used to elute bound DNA from anti-DNA antibodies. Selected fractions (*) were analyzed on a non-denaturing gel for DNA content. Boxed DNA fragment indicated is inserted (non-plasmid) DNA (CG50 or clone 11). DNA fragment size is indicated at the left and number of CpG dinucleotides is shown in parenthesis.