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Nymphaeol-A inactivates MEK1/2 and ERK1/2 phosphorylation. Tube-forming HUVECs were treated with 0.4% DMSO (0 μg/mL) or nymphaeol-A at the indicated concentrations for 6 h and 12 h, respectively. Changes in the phosphorylation state of ERK1/2 at Thr202/Tyr204 and MEK1/2 at Ser221 were analyzed by western blotting. Each experiment was repeated at least three times and representative data are shown.
