Figure 8.
Analysis of the phytochemical profile of RV extract and the inhibitory activity of fisetin on TNF-α, IL-6, and iNOS mRNA expression in LPS-treated RAW264.7 cells. (a) Phytochemical characteristics of RV extract were analyzed by high performance liquid chromatography (HPLC). (b) LPS-induced TNF-α, IL-6, and iNOS mRNA expression in fisetin-treated RAW264.7 cells was examined by quantitative ΔΔCT real-time PCR. One-way ANOVA was used for comparisons of multiple group means followed by Dunnett's t-test (# P < 0.001 versus control; *P < 0.01, **P < 0.05, ***P < 0.001 versus LPS stimulated control).