Skip to main content
. 2013 Mar 25;24(5):508–519. doi: 10.1089/hum.2012.158

FIG. 1.

FIG. 1.

pDsRed2–hepatocyte growth factor (HGF) transgene construction and in vitro validation. Vector diagram of pDsRed2-HGF transgene construct (A). HEK-293T cells transfected with pDsRed2 or pDsRed2-HGF plasmid DNA (B) show DsRed2 autofluorescence. Significant increase in HGF gene expression with the transfection of pDsRed2-HGF construct in HEK-293T cells, normalized to β-actin (C). Colocalization of DsRed2 and HGF proteins in transfected cells (D). Functional test for secreted HGF protein showed significant increase in primary rat hepatocyte proliferation (Picogreen assay) with conditioned media from pDsRed2-HGF-transfected cells (E). *p<0.05, **p<0.01.