Table 2.
Selection of matrix and phenotypical characteristics of the annulus fibrosus.
| Marker molecule | Characterisitc (Method used) |
Species | Reference |
|---|---|---|---|
| Glycosaminoglycan / Hydroxyproline ratio | Ratio 1.6:1 (biochemical assay) | Human | (Mwale et al., 2004) |
| Elastin | Co-localisation with fibrillin-1 (immunohistochemistry) |
Human; bovine |
(Yu et al., 2007) |
| Decorin, Lumican | Decreased in aged (>70 y) AF (Western Blot) |
Human | (Singh et al., 2009) |
| Collagen II / Collagen I ratio | AF<NP<AC (RT-PCR) | Rabbit | (Clouet et al., 2009) |
| Collagen V | AF>NP=AC (RT-PCR) | Rabbit | (Clouet et al., 2009) |
| Collagen I; Collagen III; Collagen V; Cadherin-13; Decorin; Versican v3 |
NP/AF<0.1 (Microarray) | Rat | (Lee et al., 2007) |
| Laminin B1; Collagen I; Collagen XIV; Aquaporin 1; CD 163; Caveolin 3; Haemoglobin beta |
NP<AF (Microarray) | Canine | (Sakai et al., 2009) |
| Tenomodulin; TNFAIP6; FOXF1; FOXF2; Aquaporin 1 |
AF>NP and AC (Microarray, RT-PCR) |
Bovine | (Minogue et al., 2010) |