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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Cancer Res. 2009 Jan 27;69(4):1293–1301. doi: 10.1158/0008-5472.CAN-08-2491

Figure 4.

Figure 4

Hyaluronan oligomers, but not hyaluronan polymer, induce internalization of CD44, CD44v3, and MCT4. A, MB231 cells were treated for 1 h with and without 100 µg/mL hyaluronan oligomer (oHA) or polymer (HA). After fixation, permeabilization, and blocking of nonspecific binding, the cells were immunolabeled for CD44 (green) and MCT4 (red) and visualized by confocal microscopy at a Z plane corresponding to the center of the cell. Treatment with hyaluronan oligomer, but not polymer, results in internalization of CD44 and MCT4 from the plasma membrane. B, MB231 cells were treated and processed as in A but were immunolabeled for CD44 (green; a, b, c) and CD44v3 (red; d, e, f). g, h, i, double labeling. Arrows, areas shown at higher magnification in j, k, and l . CD44, including CD44v3, is present at the plasma membrane in control and hyaluronan polymer-treated cells. CD44v3 seems to be internalized preferentially in response to hyaluronan oligomer treatment (h, k, and b versus e). Representative of three or more independent experiments.