FIGURE 6:

ssDNA-binding affinity of Mgm101 C-tail variants. (A) Electrophoretic mobility shift assays showing the ssDNA-binding activity of Mgm101 C-tail variants. (B) Graphic representation of the results from A. Error bars, average deviations of three independent experiments for respective C-tail variants. (C) Schematics showing the fusion of the Mgm101 C-tail with MBP. (D) SDS–PAGE showing the purification of the Mgm101 C-tail fused with MBP (MBP-C). (E) DNA-binding activity of MBP-C in comparison with the fusion of the full-length Mgm101 with MBP (MBP-Mgm101). DNA binding was performed in the absence of Mg²⁺ and in the presence of 2 mM EDTA.