FIGURE 2:

Analysis of growth and development in ndufaf5⁻. (A) Wild-type and ndufaf5⁻ cells were seeded on SM plates in association with K. aerogenes and grown for 5 d. The ndufaf5⁻ strain showed a strong defect in the diameter of the clearing zone. Bar, 1 cm. (B) Cells grown exponentially in axenic medium were harvested, washed with PDF buffer, and deposited onto nitrocellulose filters according to Materials and Methods. Photographs were taken at the indicated times. The mutant strain showed a delay at the finger-slug stage. Structures culminated after 48 h, and the fruiting bodies were smaller than those of wild type. Bar, 2 mm. Wild-type and mutant spores were taken from 10-d-old fruiting bodies and observed under phase contrast microscopy. Mutant spores were rounder and less refractive, suggesting loss of viability. Bar, 10 μm.