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. 2013 May 15;24(10):1559–1573. doi: 10.1091/mbc.E12-12-0850

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© 2013 Mitchison et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Cytokinesis midzone markers localize to pineapples. (A) Kif4 localization. A low, nonperturbing concentration of a functional Xenopus Kif4-GFP fusion (Bieling et al. 2010) was added to a pineapple assembly reaction. Shown is typical localization at 60 min (40× confocal). Kif4-GFP (red) was recruited to microtubules and accumulated at plus ends. (B) PRC1-like localization using a functional GFP fusion (see Materials and Methods). Shown is typical localization at 60 min (20× wide field). Note that PRC1L (red) localizes on the inside of the microtubule (green) structure. (C) Higher-magnification image of PRC1L localization. Shown is typical localization at 60 min (40× confocal). Note the microtubules (green) in the center of the pineapple staining for PRC1L (red). Inset, ∼2× of the colored image. (C) KIF23 (red) localization using labeled antibody raised to a C terminal peptide. Shown is typical localization at 60 min (40× confocal).