Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 15;24(10):1584–1592. doi: 10.1091/mbc.E12-08-0628

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Sasaki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Phagosomes are arrested at an RAB-7–positive stage in arl-8(tm2504) mutants. (A) AO staining of germ cell corpses in the indicated animals. Germ cell corpses are marked with arrowheads. Most cell corpses were stained by AO in arl-8(tm2504) animals, whereas corpses were not stained in ced-1(e1735) animals in which cell corpses are not phagocytosed by sheath cells. Bars, 5 μm. (B) LysoTracker staining of germ cell corpses and quantitation of LysoTracker-positive cell corpses in the indicated animals. n, number of cell corpses scored. Bars, 5 μm. (C) Time-lapse images of GFP::RAB-5 expression at the phagosome (left) and quantitation of the fluorescence intensity (right) in arl-8(tm2504)/nT1 (as a control) and arl-8(tm2504) animals. Asterisks indicate germ cell corpses. Bars, 2.5 μm. (D) Quantitation of RAB-5–positive and RAB-7–positive phagosomes in the germline of the indicated animals (left). Fifteen animals were scored for each strain, and data are expressed as mean ± SEM. **p < 0.01 by Student's t test. Nomarski and fluorescence images of arl-8(tm2504) animals expressing GFP::RAB-5 or GFP::RAB-7 (right). Arrows indicate the germ cell corpses positive for the corresponding GFP-marker proteins. Bars, 5 μm.