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. 2013 May 15;24(10):1602–1613. doi: 10.1091/mbc.E12-11-0782

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© 2013 Ueda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Knockdown of ELMO2 suppresses dendritic spine formation. (A) Cell lysates from HEK293T cells cotransfected with control or ELMO2 shRNA and HA-tagged ELMO2 were analyzed by immunoblotting with antibodies against HA and α-tubulin. (B, C) Cultured hippocampal neurons were cotransfected with EYFP and the indicated shRNA expression vector at 11 DIV and then fixed at 15 DIV. Morphology of dendrites is shown by EYFP fluorescence. Scale bar, 5 μm. (D) Under the same conditions as those in B and C, the dendritic protrusions per 10 μm of dendrite were counted. The data are mean ± SE of 45 cells in three independent experiments. ***p < 0.001.

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