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Effects of CDK6 knockdown by siRNAs. (a) RT-PCR analysis of CDK6 expression in CDK6-knocked down cells; (b) Immunoblot analysis of CDK6 and pRB protein; (c) DNA flow cytometry on cell cycle stage distribution in T98 cells harvested 48 h after transfection; (d) Viability of CDK6-knocked down cells.
