Figure 1.

Purity determination of corilagin by high pressure/performance liquid chromatography (HPLC). A: The HPLC curve of corilagin standard substance; B: The HPLC curve of corilagin sample. Purity of corilagin was determined by HPLC. A Hanbon-Kromasil 5μm C18 column was used at 30°C with the wavelength of 268nm for detection. The mobile phase was composed of 0.5% phosphoric acid and methyl cyanide with a ratio of 76:24 and the injection volume was 10μl. The purity of corilagin was calculated to 62.14%.