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. 2013 May 1;13:73. doi: 10.1186/1471-2229-13-73

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Copyright © 2013 Woldemariam et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transcript accumulation of selected genes involved in phenolamide biosynthesis in EV and MYC2-VIGS N. attenuata plants. Transcript abundance (mean ± SE; n=5) of genes involved in JA-dependent phenolamide biosynthesis was determined in WOS-induced EV and MYC2-VIGS plants. Quantification was performed by qRT-PCR using the house-keeping gene, Elongation Factor 1α (EF-1α), for normalization. One h after WOS-treatment, significant reductions (ANOVA, P < 0.05, indicated by asterisks) were observed in transcript accumulation of PAL (A), MYB8 (B), AT1 (C), CV86 (D) and DH29 (E) in MYC2-VIGS plants.