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WOS-induced accumulation of HGL-DTGs and TPI activity in EV and MYC2-VIGS plants. Leaves (n=5) of EV- and MYC2-VIGS plants were treated with WOS for 24 h, 48 h or 72 h or left untreated (0 h) and collected to extract and analyze accumulation (mean ± SE) of total HGL-DTGs (A) on HPLC-PDA. Using un-induced (0 h) and WOS-induced (24 h) samples from the same experiment, TPI activity (B) was determined using a radial diffusion assay. Asterisks indicate significant statistical differences.
