Table 4.
Antibiotic resistance genes in M.yannicii PS01 genome
| Antibiotic class | Gene name | Size (aa) | Functions | Best blast hit organism in Genbank | % aa identity | E-value |
|---|---|---|---|---|---|---|
|
Beta-lactams |
ampC |
323 |
Beta-lactamase class C |
Isoptericola variabilis 225 |
56.5 |
5.00E-114 |
|
ampC |
422 |
Beta-lactamase class C |
Microbacterium testaceum StLB037 |
54 |
1.00E-123 |
|
|
ampC |
364 |
Beta-lactamase class C |
Paenibacillus mucilaginosus KNP414 |
37.8 |
3.00E-59 |
|
|
ampC |
338 |
Beta-lactamase class C |
Arthrobacter aurescens TC1 |
41.9 |
4.00E-67 |
|
|
- |
558 |
Predicted hydrolase of the metallo-beta-lactamase superfamily |
Microbacterium laevaniformans OR221 |
88.8 |
0 |
|
|
- |
212 |
Predicted Zn-dependent hydrolases of the beta-lactamase fold |
Microbacterium testaceum StLB037 |
66.9 |
2.00E-100 |
|
|
elaC |
290 |
Metal-dependent hydrolases of the beta-lactamase superfamily III |
Saccharomonospora paurometabolica YIM 90007 |
46.6 |
2.00E-74 |
|
|
penP |
279 |
Beta-lactamase class A |
Microbacterium testaceum StLB037 |
77.3 |
7.00E-146 |
|
|
- |
615 |
Beta-lactamase domain protein |
Kribbella flavida DSM 17836 |
44.2 |
3.00E-148 |
|
|
- |
626 |
Beta-lactamase domain protein |
Mycobacterium rhodesiae JS60 |
66.8 |
0 |
|
|
- |
524 |
Zn-dependent hydrolase of the beta-lactamase fold |
Microbacterium testaceum StLB037 |
83.8 |
0.00E+00 |
|
|
Aminoglycoside |
aph |
51 |
Aminoglycoside phosphotransferase |
Microbacterium laevaniformans OR221 |
72 |
2.00E-17 |
|
- |
435 |
Predicted aminoglycoside phosphotransferase |
Microbacterium testaceum StLB037 |
61 |
3.00E-130 |
|
|
- |
292 |
Aminoglycoside phosphotransferase |
Micromonospora lupini str. Lupac 08 |
55.9 |
3.00E-95 |
|
|
- |
308 |
Aminoglycoside phosphotransferase |
Streptosporangium roseum DSM 43021 |
43.8 |
7.00E-71 |
|
|
- |
350 |
Aminoglycoside phosphotransferase |
Cellulomonas fimi ATCC 484 |
60.4 |
1.00E-125 |
|
|
Macrolides |
- |
461 |
Macrolide-efflux protein |
Beutenbergia cavernae DSM 12333 |
65.4 |
2.00E-166 |
|
Fluoroquinolones |
gyrAmutated: S83A |
883 |
DNA gyrase subunit A (EC 5.99.1.3) |
Microbacterium testaceum StLB037 |
87.7 |
0 |
|
parCmutated: S80A |
819 |
Topoisomerase IV subunit A (EC 5.99.1.-) |
Microbacterium testaceum StLB037 |
82.7 |
0 |
|
|
Sulfamides |
dhps |
281 |
Dihydropteroate synthase |
Microbacterium laevaniformans OR221 |
71.6 |
2.00E-122 |
|
Multidrug Efflux pumps |
corC |
450 |
Magnesium and cobalt efflux protein |
Microbacterium testaceum StLB037 |
78.4 |
0 |
|
kefA |
373 |
Potassium efflux system |
Microbacterium laevaniformans OR221 |
74.7 |
0 |
|
|
- |
548 |
Putative MFS Superfamily multidrug efflux transporter |
Nocardia cyriacigeorgica GUH-2 |
72.8 |
0 |
|
|
- |
513 |
putative efflux MFS permease |
Microbacterium laevaniformans OR221 |
78.8 |
0 |
|
|
- |
212 |
Putative threonine efflux protein |
Microbacterium testaceum StLB037 |
61.6 |
1.00E-80 |
|
|
- |
1275 |
RND multidrug efflux transporter; Acriflavin resistance protein |
Microbacterium laevaniformans OR221 |
78.1 |
0 |
|
|
- |
426 |
antibiotic efflux protein |
Microbacterium laevaniformans OR221 |
83.5 |
0 |
|
|
- |
526 |
probable multidrug resistance transporter, MFS family |
Cellulomonas fimi ATCC 484 |
68.8 |
0 |
|
|
- |
474 |
Inner membrane component of tripartite multidrug resistance system |
Arthrobacter aurescens TC1 |
68.2 |
0 |
|
|
- |
354 |
ABC-type multidrug transport system, ATPase component |
Saccharopolyspora erythraea NRRL 2338 |
58.8 |
1.00E-119 |
|
|
bcr/cflA |
417 |
Multidrug resistance transporter, Bcr/CflA family |
Brachybacterium paraconglomeratum LC44 |
68.5 |
1.00E-154 |
|
|
- |
519 |
multidrug resistance protein |
Arthrobacter aurescens TC1 |
54.2 |
8.00E-177 |
|
|
- |
332 |
ABC-type multidrug transport system, ATPase component |
Microbacterium laevaniformans OR221 |
72.2 |
6.00E-142 |
|
|
- |
264 |
ABC-type multidrug transport system, ATPase component |
Microbacterium testaceum StLB037 |
75 |
1.00E-143 |
|
|
- |
303 |
ABC-type multidrug transport system, ATPase component |
Paenibacillus curdlanolyticus YK9 |
59.5 |
7.00E-110 |
|
|
- |
273 |
ABC-type multidrug transport system, permease component |
Paenibacillus curdlanolyticus YK9 |
67.7 |
3.00E-121 |
|
| - | 306 | ABC-type multidrug transport system, ATPase component | Clavibacter michiganensis subsp. michiganensis NCPPB 382 | 60.8 | 3.00E-107 |
General features of CF M. yannicii PS01 resistome showing the antibiotic resistance genes present and percentage of identity with best blast hit organism.