Figure 1. In vitro system for differentiation of hematopoietic cells.

A. In vitro differentiation of un-fractionated CD34+ progenitors co-cultured on a stromal monolayer in medium containing 5% LPA-depleted serum in the presence of thrombopoietin, Flt3 Ligand and IL-7, cytokines that allow generation of both myeloid (CD45+CD14+ monocytes, CD45+CD66b granulocytes and CD45^negCD41a+ megakaryocytes) and B lymphoid cells: CD45+CD10+ CD19neg or CD45+CD10+CD19+. Control panel represents unstained cells. B. Autotaxin protein expression in commonly used stromal lines: MS5, OP9 and human bone marrow-derived mesenchymal stromal cells. Positive signal is shown in brown color (DAB). Magnification 20X. Images were acquired using the Zeiss Axiovision software version 4.8 Carl Zeiss Microscope (Carl Zeiss, Germany).