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. 2013 May 16;8(5):e63966. doi: 10.1371/journal.pone.0063966

Figure 4. DTCD mediates transcription of DR5 through Sp1 activation.

Figure 4

(A) DTCD treatment increases the DR5 mRNA levels. Real-time PCR was used to quantify the DR5 mRNA levels following 24 h of DTCD treatment. (B) Effects of DTCD on DR5 promoter activity. pDR5/−605 plasmid was transfected into A2780 cells, which were then treated with DTCD (10 µM) for the indicated time points or indicated amounts of DTCD, lysed and assayed for luciferase activity. (C) DTCD activates Sp1 translocation to the nucleus. (D) In vivo binding of Sp1 on DR5 promoter regions by DTCD. ChIP assay was done using antibodies against Sp1 in both cells. Negative controls were done using antibody against rabbit IgG.