Table 1. Comparison of Cq values (Mean ±1 SD; n = 9) from single and duplex real-time PCR assays obtained with dilution series of Borrelia DNA or a synthetic Borrelia 16S oligonucleotide in a background matrix of human genomic DNA.

Copies 16S	Synthetic Borrelia 16S		Total Borrelia DNA
	16S	16S/RNAseP	16S	16S/RNAseP
10,000	26.37±0.28	26.36±0.20	26.44±0.11	26.46±0.11
1,000	29.78±0.22	29.74±0.19	29.89±0.12	29.87±0.06
100	33.17±0.34	33.32±0.24	33.42±0.32	33.12±0.20
10	36.58±0.46	37.03±1.23	37.00±0.77	36.58±0.55
5	37.30±0.72	37.83±1.16	38.33±1.17	37.74±0.85
2.5	38.84±1.25	38.37±0.84	39.02±1.01	38.45±0.84
1	39.49±0.80	39.10±0.98	39.66±0.58	39.52±0.63
PCR Efficiency	104% ±4%	104% ±6%	98% ±1%	103% ±3%
R2	0.99±0.004	0.98±0.01	0.98±0.15	0.99±0.004
Y-Intercept	39.45±0.24	39.48±0.27	40.03±0.19	39.06±1.14
Slope	−3.23±0.08	−3.22±0.13	−3.37±0.12	−3.25±0.06