Table 10. Details of primers used for qRT-PCR analysis and genotyping.
| Gene | Reference ID | Primer sequences (5′→3′) | Application | Position* | Enzyme | Annealing temperature (°C) | Product size (bp) | RFLP-patterns |
| CYP2B22 | NM_214413.1 | F: CACCACCATCCTCCAGAACT R: GGCAGGAACTGGATCTGGTA | qRT-PCR | – | – | 52 | 120 | – |
| ARG2 | XM_001928679.2 | F: GGAAGCTGGCTTGATGAAAA R: CCACTGAGCGAGGATTCACT | qRT-PCR | – | – | 55 | 128 | – |
| MSMO1 | NM_213752.1 | F: CCTGGCACTATTTCCTGCAT R: TAGGGTTTCCAGAGGGTGTG | qRT-PCR | – | – | 55 | 128 | – |
| EDN1 | NM_213882.1 | F: TTCAGGGAGAAACACCCAAG R:CGAGACGGAAGAAAGCAAAG | qRT-PCR | – | – | 55 | 121 | – |
| CYP2C33 | NM_214414.1 | F: AGCTGTGCCTCATCCCTAGA R: GTGTTTCTGTCCCAGGCAAT | qRT-PCR | – | – | 56 | 133 | – |
| IP6K1 | XM_001925759.2 | F: CTGCCAGCCTGTGTCTGTAA R: ATGGCACCAGAATCAGAAGG | qRT-PCR | – | – | 55 | 136 | – |
| BTG3 | XM_003132741.1 | F: CCAGGAATGTACCGAGGAAA R: ACAATGCATTCCAGGAGGAG | qRT-PCR | – | – | 55 | 138 | – |
| CYP7A1 | NM_001005352.2 | F: TTCCCGATTCATGTGTTCAA R: ACCAGTTCCGAGATGTGGTC | qRT-PCR | – | – | 54 | 104 | – |
| FMO5 | XM_001928594.1 | F:GGCCTGAAGCCTAAACACAG R:CCTGGAGCCATCCTCAAATA | qRT-PCR | – | – | 55 | 147 | – |
| HSD17B2 | NM_001167649.1 | F: TGCAGAACAGAGGACTGTGG R: GCCATGCATCGTTTGTATTG | qRT-PCR | – | – | 54 | 103 | – |
| PPIA | NM_214353 | F: CACAAACGGTTCCCAGTTT R: TGTCCACAGTCAGCAATGGT | qRT-PCR | – | – | 58 | 171 | – |
| GAPDH | AF017079 | F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC | qRT-PCR | – | – | 60 | 247 | – |
| IRG6 | NM_213817 | F: CTGGTACCTGTCACCTTTGC R: GGGTGAAGTGGTAATTGACG | Genotyping | Exon 3 | HaeIII | 60 | 232 | GG: 154+78AA: 232 |
| DSP | XM_003128168 | F: AACCTGATTGATCGGGAAAC R: GCTGACCTTCTTTTTGGTGA | Genotyping | Exon 1 | HpyCH4IV | 55 | 207 | CC: 109+98TT: 207 |
| MX1 | NM_214061 | F:CACTTCCAAATGGAGCAGAT R:GACTCGCAGACTCACCTGAT | Genotyping | Exon 2 | AciI | 55 | 204 | CC: 125+79TT: 204 |
| IFIT2 | XM_001928671 | F:AAGAAGTTTTCCAGCCCCTA R:TTATCCAGACGGTAGCTTGC | Genotyping | Exon 3 | DrdI | 55 | 188 | GG: 145+107TT: 252 |
| FMO5 | XM_001928594 | F:AAAGGTTCGACCATGAAATG R: TATGGCAGCTGTCTCTGTGA | Genotyping | Exon 3 | HpyCH4III | 55 | 223 | GG: 134+89AA: 223 |
| CYP7A1 | NM_001005352 | F:TGTCCAGGAAATCAAGCAAT R:CGTCATCAGCTGTCCTCTTT | Genotyping | Exon 2 | HpyCH4V | 55 | 199 | AA: 101+98GG: 199 |
| KRT18 | NM_001159615 | F: GGGTTGAGAAGGTTCTGGAT R: CTCCTCGTGGTTCTTCTTCA | Genotyping | Exon 2 | HpyCH4V | 55 | 215 | GG: 149+66AA: 215 |
*
Position according to coding region in Sus scrofa.